<div dir="ltr"><div class="gmail_quote"><div dir="ltr"><div>Desde la Unidad de Microscopía del Institut Pasteur de Montevideo tenemos el agrado de invitarlos a las charlas que dará el
Prof. Dr. Ulrich Kubitscheck en el Institut Pasteur de Montevideo el
próximo jueves 10 y viernes 11 de marzo a las 11:00 horas. <br><div class="gmail_quote"><br><div dir="ltr">El
Dr. Kubitscheck es el responsable del Biophysical Chemistry Workgroup,
Institute of Physical and Theoretical Chemistry, University of Bonn,
Alemania. Es el editor del libro Fluorescence Microscopy: From
Principles to Biological Applications<br><br>A continuación se detallan las dos charlas que realizará. Agradecemos la difusión de estas charlas.<br><br>Saludos cordiales, <br><br>Flavio Zolessi<br>Federico Lecumberry<br><br><br><b> "Light Sheet Fluorescence Microscopy: A Pathway into New Worlds"<br>Jueves 10 de marzo 11:00 horas, Institut Pasteur de Montevideo<br></b>Ulrich Kubitscheck<br><br>Light
sheet fluorescence microscopy is a relatively young light microscopic
technique, which features optical sectioning, low photo-toxicity and
rapid image acquisition. The technique is based on illuminating the
sample orthogonally to the detection pathway with a thin, focused sheet
of light. The basic principle of the method will be introduced, and
current lines of methodological developments in our lab will be
outlined: confocal light sheet imaging, 2-photon excitation, large
volume imaging and combination with expansion microscopy.<br><br><b><br></b><b> "Real Time Observation of Single Molecule Pathways in Biological Systems"<br>Viernes 11 de marzo 11:00 horas, Institut Pasteur de Montevideo<br></b>Ulrich Kubitscheck, Tim Kaminski, Katharina Scherer, Jan-Hendrik Spille, and Jan Peter Siebrasse*<br><br>Observation
and tracking of fluorescently labeled molecules and particles in living
cells reveals detailed information about intracellular processes on the
molecular level, e.g. the nuclear export of RNA particles (1). Whereas
light microscopic particle observation is usually limited to
two-dimensional projections of short trajectory segments, we report here
image-based real-time three-dimensional single particle tracking in an
active feedback loop with single molecule sensitivity. We tracked
particles carrying only 1–3 fluorophores deep inside living tissue with
high spatio-temporal resolution (2). Using this approach, we succeeded
to acquire trajectories containing several hundred localizations. We
present statistical methods to find significant deviations from random
Brownian motion in such trajectories. The analysis allowed us to
directly observe transitions in the mobility of ribosomal (r)RNA and
Balbiani ring (BR) messenger (m)RNA particles in living Chironomus
tentans salivary gland cell nuclei. We found that BR mRNA particles
displayed phases of reduced mobility, while rRNA particles showed
distinct binding events in and near nucleoli.<br><br>(1) Siebrasse.
J.P., T.Kaminski and U.Kubitscheck. 2012. Nuclear export of single
native mRNA molecules
observed by light sheet fluorescence microscopy.
Proc Natl Acad Sci USA 109: 9426-31. <br>(2) Spille, J.-H., T.P. Kaminski,
K. Scherer, J.S. Rinne, A. Heckel, and U. Kubitscheck. 2014 Direct
observation of mobility state transitions in RNA trajectories by
sensitive single molecule feedback tracking. Nucleic Acids Res. 2014;
doi: 10.1093/nar/gku1194. <br>(3) Spille, J.-H. and U. Kubitscheck. 2015.
Labelling and imaging of single endogenous messenger RNA particles in
vivo. J. Cell Sci 128(20):3695-706.<span><font color="#888888"><br><br></font></span><br></div></div></div></div>
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