[DPS-seminario] Invitación a charlas del Dr. Ulrich Kubitscheck en Microscopía de Fluorescencia

[federico lecumberry]

Desde la Unidad de Microscopía del Institut Pasteur de Montevideo tenemos
el agrado de invitarlos a las charlas que dará el Prof. Dr. Ulrich
Kubitscheck en el Institut Pasteur de Montevideo el próximo jueves 10 y
viernes 11 de marzo a las 11:00 horas.

El Dr. Kubitscheck es el responsable del Biophysical Chemistry Workgroup,
Institute of Physical and Theoretical Chemistry, University of Bonn,
Alemania. Es el editor del libro Fluorescence Microscopy: From Principles
to Biological Applications

A continuación se detallan las dos charlas que realizará. Agradecemos la
difusión de estas charlas.

Saludos cordiales,

Flavio Zolessi
Federico Lecumberry




* "Light Sheet Fluorescence Microscopy: A Pathway into New Worlds"Jueves 10
de marzo 11:00 horas, Institut Pasteur de Montevideo*Ulrich Kubitscheck

Light sheet fluorescence microscopy is a relatively young light microscopic
technique, which features optical sectioning, low photo-toxicity and rapid
image acquisition. The technique is based on illuminating the sample
orthogonally to the detection pathway with a thin, focused sheet of light.
The basic principle of the method will be introduced, and current lines of
methodological developments in our lab will be outlined: confocal light
sheet imaging, 2-photon excitation, large volume imaging and combination
with expansion microscopy.




* "Real Time Observation of Single Molecule Pathways in Biological
Systems"Viernes 11 de marzo 11:00 horas, Institut Pasteur de Montevideo*Ulrich
Kubitscheck, Tim Kaminski, Katharina Scherer, Jan-Hendrik Spille, and Jan
Peter Siebrasse*

Observation and tracking of fluorescently labeled molecules and particles
in living cells reveals detailed information about intracellular processes
on the molecular level, e.g. the nuclear export of RNA particles (1).
Whereas light microscopic particle observation is usually limited to
two-dimensional projections of short trajectory segments, we report here
image-based real-time three-dimensional single particle tracking in an
active feedback loop with single molecule sensitivity. We tracked particles
carrying only 1–3 fluorophores deep inside living tissue with high
spatio-temporal resolution (2). Using this approach, we succeeded to
acquire trajectories containing several hundred localizations. We present
statistical methods to find significant deviations from random Brownian
motion in such trajectories. The analysis allowed us to directly observe
transitions in the mobility of ribosomal (r)RNA and Balbiani ring (BR)
messenger (m)RNA particles in living Chironomus tentans salivary gland cell
nuclei. We found that BR mRNA particles displayed phases of reduced
mobility, while rRNA particles showed distinct binding events in and near
nucleoli.

(1) Siebrasse. J.P., T.Kaminski and U.Kubitscheck. 2012. Nuclear export of
single native mRNA molecules observed by light sheet fluorescence
microscopy.  Proc Natl Acad Sci USA 109: 9426-31.
(2) Spille, J.-H., T.P. Kaminski, K. Scherer, J.S. Rinne, A. Heckel, and U.
Kubitscheck. 2014 Direct observation of mobility state transitions in RNA
trajectories by sensitive single molecule feedback tracking. Nucleic Acids
Res. 2014; doi: 10.1093/nar/gku1194.
(3) Spille, J.-H. and U. Kubitscheck. 2015. Labelling and imaging of single
endogenous messenger RNA particles in vivo. J. Cell Sci 128(20):3695-706.
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